Difference between revisions of "Part:BBa K323214:Experience"

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Results obtained by testing [https://parts.igem.org/Part:BBa_K782011#Characterization NicTAL12:KRAB] repressor proved that NicTAL12 binds to its binding sites better than NicTAL10:KRAB.
 
  
 
[[Image:Svn_12_NicTAL_graf.png]]
 
[[Image:Svn_12_NicTAL_graf.png]]

Revision as of 10:59, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K323214

User Reviews

[http://2012.igem.org/Team:Slovenia 2012 iGEM team Slovenia ] found out that NicTAL part deposited in the Registry by the Slovenian iGEM2010 team was missing subdomain in DNA-binding domain, so our team added this missing part on N terminal sequence. Apart from adding N terminal sequence, we added HIS tag on N terminal end and NLS on terminal C end of NicTAL12 (Figure 1). This construct was later used for designing TAL-based activator and repressor by adding VP16 and KRAB domain.

Svn 12 NicTAL10vs12.PNG

Figure 1:Sequence alignment of NicTAL10 and NicTAL12, showing differences between constructs.


Svn 12 NicTAL graf.png

Figure 2: Results of testing NicTAL:KRAB repressor demonstrated better binding ability of NicTAL12.



UNIQ21af6acecb394cf5-partinfo-00000000-QINU UNIQ21af6acecb394cf5-partinfo-00000001-QINU