Difference between revisions of "Part:BBa K823028:Design"

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===References===
 
===References===
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Reference:[http://www.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ Promega]

Latest revision as of 10:15, 26 September 2012

luc luciferase + RBS, codon optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 625
    Illegal SapI site found at 853


Design Notes

codon optimized for Bacillus subtilis

prefix: (next nucleotide is then the a from atg); Includes RBS with correct spacing

GAGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT

suffix: (directly after the TAA)


TACTAGTAGCGGCCGCTGCAGGCTGA

Source

synthesis by gene art

References

Reference:[http://www.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ Promega]