Difference between revisions of "Part:BBa K782067"

Revision as of 10:14, 26 September 2012

2x[NicTAL]+2x[TALD] operator

• TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contains two specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC

Figure 1. Shematic representation of two consecutive specific binding sites for NicTAL12 and TALD.

• Binding sites for TAL effectors were ordered from IDT.

Characterization

Specific TAL binding sites were further characterized with different reporter constructs.

Results:

BBa_K782001

References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698

Sequence and Features