Difference between revisions of "Part:BBa K782067"

Revision as of 10:14, 26 September 2012

2x[NicTAL]+2x[TALD] operator

TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contains two specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC

Figure 1. Shematic representation of two consecutive specific binding sites for NicTAL12 and TALD.

Binding sites for TAL effectors were ordered from IDT.

Characterization

Specific TAL binding sites were further characterized with different reporter constructs.

Results: BBa_K782001

References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 35
Illegal AgeI site found at 105
1000
COMPATIBLE WITH RFC[1000]

@@ Line 18: / Line 18: @@
 '''Figure 1.''' Shematic representation of two consecutive specific binding sites for NicTAL12 and TALD.
+* Binding sites for TAL effectors were ordered from IDT.
 ==Characterization==
+Specific TAL binding sites were further characterized with different reporter constructs.
-Results: Specific TAL binding sites were further characterized with different reporter constructs.
+'''Results:'''
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K782001 BBa_K782001]
-* Binding sites for TAL effectors were ordered from IDT.