Difference between revisions of "Part:BBa K823039"

Revision as of 09:37, 26 September 2012

gfpmut1 (B. subtilis optimized + Freiburg standard) This gfpmut3 gene was amplified from pGFPamy B. subtilis vector.

It is constructed in the Freiburg standard with RBS.

Find out more about the design of our prefix with ribosome binding site.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

We took a gfp derivate of the Bacillus subtilis plasmids pGFPamy and added the BioBrick compatiple pre- and suffix of the Freiburg standard (Assembly 25). This BioBrick was used to create our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads]. It is part of the BioBrick K823049

Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus BioBrick Box] and the created [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 197

@@ Line 11: / Line 11: @@
 suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
-<p align="justify">[[Image:LMU Firstspore.jpg|200px|left]]We took a ''gfp'' derivate of the ''Bacillus subtilis'' plasmids pGFPamy and added the BioBrick compatiple pre- and suffix of the Freiburg standard (Assembly 25). This BioBrick was used to create our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads].</p>
+<p align="justify">[[Image:LMU Firstspore.jpg|200px|left]]We took a ''gfp'' derivate of the ''Bacillus subtilis'' plasmids pGFPamy and added the BioBrick compatiple pre- and suffix of the Freiburg standard (Assembly 25). This BioBrick was used to create our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads]. It is part of the BioBrick [https://parts.igem.org/Part:BBa_K823049 K823049]</p>
 Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks ''<b>Bacillus'' B</b>io<b>B</b>rick <b>B</b>ox] and the created [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads].