Difference between revisions of "Part:BBa K934022:Experience"

Revision as of 09:33, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K934022

We accomplished a positive feedback system with our improved part Plux-LasI (BBa_K934012).

File:Positivefeedback result.png

By co-culturing Plux-LasI cell and Plas-LuxI cell, we confirmed higher concentration of a signal than initial conditions was detected through production of the other signal (red arrow & blue arrow). This result strongly suggests that our positive feedback system worked accurately.

User Reviews

UNIQbc44318c9deebf53-partinfo-00000000-QINU UNIQbc44318c9deebf53-partinfo-00000001-QINU

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 ===Applications of BBa_K934022===
-[[Image:Plux-LasI_result.png|thumb|center|500px|]]
-To characterize Plux-LasI (BBa_K934022), we introduced Plux-LasI (BBa_K934022) with Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) to E.coli as “3OC6HSL dependent 3OC12HSL producer cell”. In this E.coli, constitutively expressed LuxR activates the expression of LasI in the presence of 3OC6HSL. We then introduced Ptrc-LasR  and Plas-GFP ([https://parts.igem.org/Part:BBa_K649001 BBa_K649001]) to E.coli as a “Las reporter cell”.
-In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI (BBa_K934022) synthesized 3OC12HSL.
 We accomplished a positive feedback system with our improved part Plux-LasI ([https://parts.igem.org/Part:BBa_K934012 BBa_K934012]).