Difference between revisions of "Part:BBa K934012:Experience"

Revision as of 09:28, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K934012

We accomplished a positive feedback system with our new Plux-LasI (BBa_K934022).

File:Positivefeedback result.png

By co-culturing Plux-LasI cell and Plas-LuxI cell, we confirmed higher concentration of a signal than initial conditions was detected through production of the other signal (red arrow & blue arrow). This result strongly suggests that our positive feedback system worked accurately.

User Reviews

UNIQ6d7c85a39e9e1634-partinfo-00000000-QINU UNIQ6d7c85a39e9e1634-partinfo-00000001-QINU

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 [[Image:Plas-LuxI_result.png|thumb|center|500px|]]
-To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to E.coli as a “Lux reporter cell”.
-In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI (BBa_K934012) synthesized 3OC6HSL.
 We accomplished a positive feedback system with our new Plux-LasI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]).