Difference between revisions of "Part:BBa K819008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | BL21 cells harboring T7-lux operon induced with IPTG at 5*10<sup>-5</sup>M is shown in the photo<br/><br/> | + | BL21 cells harboring T7-lux operon induced with IPTG at 5*10<sup>-5</sup>M is shown in the photo<br/>The best temperature for cells to glow is 30<sup>o</sup>C rather than 37<sup>o</sup>C.<br /><br/> |
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<img src="https://static.igem.org/mediawiki/parts/0/02/Peking2012_part_T7_lux_operon.png" style="width:200px;"/> | <img src="https://static.igem.org/mediawiki/parts/0/02/Peking2012_part_T7_lux_operon.png" style="width:200px;"/> | ||
Revision as of 09:27, 26 September 2012
Lux Operon under T7 promoter
Lux operon genes (from BBa_K325909) and related RBS are placed under T7 promoter. Cells transformed with this part can produce blue luminescence while no exogenous substrate is needed.
luxbrick under T7 promoter is very modular, because it is transcribed by T7 polymerase, which can be placed under any other promoter, forming a interface between luxbrick and other systems.Also, when transformed into BL21 cells, it can be induced with IPTG to reach a high expression level.
Usage and Biology
BL21 cells harboring T7-lux operon induced with IPTG at 5*10-5M is shown in the photo
The best temperature for cells to glow is 30oC rather than 37oC.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726