Difference between revisions of "Part:BBa K266000:Experience"
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| − | + | Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Also, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Parts Experience page of the Registry. Thus, in this study, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part. | |
| − | + | [[Image:Plas-LuxI_result.png|thumb|center|500px|]] | |
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Revision as of 09:26, 26 September 2012
Documentation:The image of the plasmid of this biobrick can be found in [http://2009.igem.org/Image:AI_Biobricks4.jpg (1)] and [http://2009.igem.org/Image:AI_Biobricks4.jpg (2)] with a gel analysis confirming dna size.
Applications of BBa_K266000
In the [http://2009.igem.org/Team:IPN-UNAM-Mexico/Parts IPN-UNAM MEXICO PROJECT]
User Reviews
UNIQ833f0c10844be8ec-partinfo-00000000-QINU
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Tokyo-tech iGEM 2012 |
Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Also, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Parts Experience page of the Registry. Thus, in this study, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.
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UNIQ833f0c10844be8ec-partinfo-00000002-QINU