Difference between revisions of "Part:BBa K823022:Design"

 
 
Line 1: Line 1:
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823022 short</partinfo>
 
<partinfo>BBa_K823022 short</partinfo>
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===Source===
 
===Source===
  
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B.subtilis vector pDG1731
+
''B. subtilis'' vector pDG1731
  
 
===References===
 
===References===
 +
 +
[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']

Latest revision as of 08:43, 26 September 2012

pSBBs4S: Empty backbone for integration into Bacillus subtilis thrC locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4552
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4552
    Illegal BamHI site found at 4534
    Illegal XhoI site found at 1011
    Illegal XhoI site found at 1929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4552
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4552
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4567
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1893
    Illegal NgoMIV site found at 3032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2856
    Illegal SapI.rc site found at 605


Design Notes

This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.


Source

B. subtilis vector pDG1731

References

[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]