Difference between revisions of "Part:BBa K823019:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823019 short</partinfo>
 
<partinfo>BBa_K823019 short</partinfo>
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===Source===
 
===Source===
  
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Bacillus subtilis reporter vector pAC6.
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''Bacillus subtilis'' reporter vector pAC6.
  
 
===References===
 
===References===
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[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]

Latest revision as of 08:42, 26 September 2012

LacZ (RBS+Freiburg standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed the part in the Freiburg standard (assembly 25) for inframe fusion-proteins. There is a Shine-Dalgarno Sequence included between the XbaI and the NgoIV restriction site. It can be used for both N and C terminal fusions.


Source

Bacillus subtilis reporter vector pAC6.

References

[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]