Difference between revisions of "Part:BBa K823042"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823042 short</partinfo> | <partinfo>BBa_K823042 short</partinfo> | ||
| − | A B.subtilis promoter regulated by sigma G late in Sporulation in the forespore | + | A ''B. subtilis'' promoter regulated by sigma G late in Sporulation in the forespore |
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| + | [[Image:SuicideSwitchgrafik.jpg|600px]] | ||
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| + | Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 PsspK],[https://parts.igem.org/Part:BBa_K823043 ECF41] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch <b>Suicide</b> switch]. | ||
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| + | Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:18, 26 September 2012
PsspK promoter regulated by sigma G
A B. subtilis promoter regulated by sigma G late in Sporulation in the forespore
Our team used this part in the combination with PspoIVB/PsspK,ECF41 and MazF in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].
Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]