Difference between revisions of "Part:BBa K323214:Experience"
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| − | '''Figure 2:''' Results of testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782011 | + | '''Figure 2:''' Results of testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782011 NicTAL:KRAB] repressor demonstrated better binding ability of NicTAL12. |
Revision as of 08:09, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K323214
User Reviews
[http://2012.igem.org/Team:Slovenia 2012 iGEM team Slovenia ] found out that NicTAL part deposited in the Registry by the Slovenian iGEM2010 team was missing subdomain in DNA-binding domain, so our team added this missing part on N terminal sequence. Apart from adding N terminal sequence, we added HIS tag on N terminal end and NLS on terminal C end of NicTAL12 (Figure 1). This construct was later used for designing TAL-based activator and repressor by adding VP16 and KRAB domain.
Figure 1:Sequence alignment of NicTAL10 and NicTAL12, showing differences between constructs.
Results obtained by testing NicTAL12:KRAB repressor proved that NicTAL12 binds to its binding sites better than NicTAL10:KRAB.
Figure 2: Results of testing NicTAL:KRAB repressor demonstrated better binding ability of NicTAL12.
UNIQaff057ed9f13ec1d-partinfo-00000000-QINU UNIQaff057ed9f13ec1d-partinfo-00000001-QINU