Difference between revisions of "Part:BBa K782072"

Revision as of 08:06, 26 September 2012

12x[TALD] operator

• TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contain twelve specific binding sites for TALD (Figure 1).

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC

Figure 1. Shematic representation of the construct.

• Binding sites for TAL effectors were ordered from IDT.

References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698

Sequence and Features