Difference between revisions of "Part:BBa K786010"
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| − | This part contains the otnpF promoter, after the stimulation of light, Sensory Rhodopsins are able to control the the autophosphorylation activity of CheA. While CheA can phosphorylate OmpR and phosphorylated OmpR is able to stimulate transcription from the otnpF promoter (part R0083). | + | This part contains the otnpF promoter, after the stimulation of light, Sensory Rhodopsins are able to control the the autophosphorylation activity of CheA. While CheA can phosphorylate OmpR and phosphorylated OmpR is able to stimulate transcription from the otnpF promoter (part R0083).Must be used in E.coli deficient in EnvZ. |
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Revision as of 07:57, 26 September 2012
Gene expression for sensory rhodopsin I & II (SRI & SRII)
This part contains the otnpF promoter, after the stimulation of light, Sensory Rhodopsins are able to control the the autophosphorylation activity of CheA. While CheA can phosphorylate OmpR and phosphorylated OmpR is able to stimulate transcription from the otnpF promoter (part R0083).Must be used in E.coli deficient in EnvZ.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 659
Illegal AgeI site found at 771 - 1000COMPATIBLE WITH RFC[1000]