Difference between revisions of "Part:BBa K819010"

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<img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/>
 
<img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/>
<p>Figure 1. </p>
 
 
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<p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 ([https://parts.igem.org/Part:BBa_K819009 BBa_K819009]). <b>c)cheZ gene under medium promoter BBa_J23113 (this part)</b> d)wild type MG1655 strain</p>
 
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Revision as of 07:23, 26 September 2012

Medium cheZ generator

CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.

Figure 1. E.coli was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 (BBa_K819009). c)cheZ gene under medium promoter BBa_J23113 (this part) d)wild type MG1655 strain


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]