Difference between revisions of "Part:BBa K769019"

Latest revision as of 07:18, 26 September 2012

Promoter(high)-lumP-DT

BBa_K769011+Promoter(high)-lumP-DT

lumazine protein

Bacterial luciferase derived from Vibrio fischeri, which is a strain of luminous bacteria, shows brue-green light emission (wavelength is 495 nm). however, in luminous bacteria, there are strains that emit light with shorter wavelength than normal Vibrio fischeri (475nm,blue), such as Photobacterium phosphoreum and Photobacterium leiognathi. It is known that blue shift of these luminous bacterium is caused by lumazine protein (LumP). LumP shifts the wavelength by interacting with the luciferase. As a chromophore, it has 6,7-dimethyl-8-(1'-D-ribityl)lumazine (DMRL), and its size is about 21kDa.

Learning from previous reports, we decided to use these proteins for changing the color.

Improve the existing BioBrick Part

We used BioBrick part(BBa_K216007) coding lumazine protein. We thought that gene coding protein only is difficult to use for constructing device, so we added promoter and double terminator for this part.

Sequence and Features