Difference between revisions of "Part:BBa K934012"

Revision as of 07:17, 26 September 2012

Plas-LuxI

We constructed this part by combining BBa_K649000 and BBa_K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.

In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL.

We improved a previous part Plas-LuxI (BBa_K266000) and accomplished a positive feedback system with our new Plux-LasI (BBa_K934022).

For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 749
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL.
-We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000 BBa_K266000])
+We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000:Experience#User_Reviews BBa_K266000])
 and accomplished a positive feedback system with our new Plux-LasI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]).