Difference between revisions of "Part:BBa K819010"
Zhangzidong (Talk | contribs) |
Zhangzidong (Talk | contribs) |
||
Line 4: | Line 4: | ||
CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | ||
CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23113 BBa_J23113]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain. | CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23113 BBa_J23113]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain. | ||
+ | |||
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/> | ||
+ | <p>Figure 1. </p> | ||
+ | </html> | ||
+ | <br /> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 06:49, 26 September 2012
Medium cheZ generator
CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
Figure 1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]