Difference between revisions of "Part:BBa K819012"

Revision as of 04:37, 26 September 2012

Luminesensor repressible LacIM generator

LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor BBa_K819005 constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K819012 short</partinfo>
-LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
+LacI mutant protein is placed under SulA408 promoter<br />
+SulA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819005 BBa_K819005] constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in <i>E.coli</i>.
+<br />LacI mutant from [https://parts.igem.org/Part:BBa_K395400 BBa_K395400] can repress lac promoter. So this part can work as a connection between two systems.
 <!-- Add more about the biology of this part here