Difference between revisions of "Part:BBa K750002"

Latest revision as of 01:43, 26 September 2012

LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

This part contains pBAD promoter, rbs of 0.6 strength, LuxI producer and double terminator. Once the promoter PBADis activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 796
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 01:22, 26 September 2012 (view source) AlexYao (Talk \| contribs) ← Older edit		Latest revision as of 01:43, 26 September 2012 (view source) Ishuidi (Talk \| contribs)
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	<partinfo>BBa_K750002 short</partinfo>		<partinfo>BBa_K750002 short</partinfo>

−	This part contains pBAD promoter, rbs of 0.6 strength, LuxI producer and double terminator. Once the promoter ~~Pbad is~~ activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project.	+	This part contains ''pBAD'' promoter, rbs of 0.6 strength, LuxI producer and double terminator. Once the promoter PBADis activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project.