Difference between revisions of "Part:BBa K750115"
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<partinfo>BBa_K750115 short</partinfo> | <partinfo>BBa_K750115 short</partinfo> | ||
| − | This part contains pBADcIT(BBa_K750104),PtetcIT(BBa_K750102),PcIGLT(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from PcIGLT. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project. | + | This part contains ''pBADcIT''(BBa_K750104),''PtetcIT''(BBa_K750102),''PcIGLT''(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from ''PcIGLT''. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project. |
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Latest revision as of 01:29, 26 September 2012
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)
This part contains pBADcIT(BBa_K750104),PtetcIT(BBa_K750102),PcIGLT(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from PcIGLT. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1083
Illegal NheI site found at 1106 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3686