Difference between revisions of "Part:BBa K802009:Design"

(→‎References)
(→‎References)
Line 15: Line 15:
  
 
===References===
 
===References===
−
1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007;
+
1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007; <br>
 
2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on
 
2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on
−
pharmamedia commercial medium (2007);
+
pharmamedia commercial medium (2007);<br>
 
3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin
 
3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin
 
Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005
 
Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005

Revision as of 01:19, 26 September 2012

Sufactin generator and biofilm repressor for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760
    Illegal SapI.rc site found at 770


Design Notes

The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible Pxyl promoter.


Source

The part was constructed from the assembly of 3 biobricks: BBa_K802006,BBa_K802007 and BBa_K802008.

References

1. Fernandes, P.A.V. et al., Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria, Braz. J. Microbiol. vol.38 no.4 São Paulo Oct./Dec. 2007;
2. Al-Ajlani, M.M. et al., Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium (2007);
3. Abushadi, H.M. et al., Molecular Characterization of Bacillus subtilis Surfactin Producing Strain and the Factors Affecting its Production, Int. J. Agri. Biol., Vol. 7, No. 3, 2005