Difference between revisions of "Part:BBa K750008:Design"

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===Design Notes===
 
===Design Notes===
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When we constructed TD1.0, we used two different methods: one is to ligate LuxR producer and GFP reporter,and then ligated with LuxI producer; another is to ligate LuxI producer and LuxR producer first, and then ligated with GFP reporter.
+
When we constructed TD1.0, we used two different methods: one is to ligate LuxR producer and GFP reporter,and then ligated with LuxI producer; another is to ligate LuxI producer and LuxR producer first, and then ligated with GFP reporter. This part was designed for last method.
  
 
===Source===
 
===Source===

Revision as of 01:01, 26 September 2012

Quorum sensing system based on LuxI and LuxR to control the expression of parts behind


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1069
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 793
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we constructed TD1.0, we used two different methods: one is to ligate LuxR producer and GFP reporter,and then ligated with LuxI producer; another is to ligate LuxI producer and LuxR producer first, and then ligated with GFP reporter. This part was designed for last method.

Source

We built this part by biobricks from the DNA distribution kit plates 2012

References

[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.

[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.

[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.

[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.

[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.

[6]http://2011.igem.org/Team:XMU-China