Difference between revisions of "Part:BBa K934025"
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<partinfo>BBa_K934025 short</partinfo>
 
<partinfo>BBa_K934025 short</partinfo>
  
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We constructed this part by ligating Plux/tet hybrid promoter ([https://parts.igem.org/Part:BBa_K934025 BBa_K934025]) to the upstream of promoterless GFP generator ([https://parts.igem.org/Part:BBa_K934025 BBa_I13504]).
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We constructed this part by ligating Plux/tet hybrid promoter ([https://parts.igem.org/Part:BBa_K934024 BBa_K934024]) to the upstream of promoterless GFP generator ([https://parts.igem.org/Part:BBa_K934025 BBa_I13504]).
  
 
[[Image:Pluxtet_assay.png|thumb|center|500px|]]
 
[[Image:Pluxtet_assay.png|thumb|center|500px|]]

Revision as of 00:50, 26 September 2012

Plux/tet-GFP

We constructed this part by ligating Plux/tet hybrid promoter (BBa_K934024) to the upstream of promoterless GFP generator (BBa_I13504).

Pluxtet assay.png

In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751