Difference between revisions of "Part:BBa K750006:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
This time, we desided to just change the RBS of LuxI producer while continued to use RBS1.0 in LuxR producer.Maybe next time we can make something changed in LuxR producer.
+
This time, we desided to just change the RBS of LuxI producer while continued to use RBS1.0 in LuxR producer.Maybe next time we can change something in LuxR producer.
  
 
===Source===
 
===Source===

Latest revision as of 00:49, 26 September 2012

LuxR expression device activated by arabinose


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This time, we desided to just change the RBS of LuxI producer while continued to use RBS1.0 in LuxR producer.Maybe next time we can change something in LuxR producer.

Source

We built this part by biobricks from the DNA distribution kit plates 2012

References

[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.

[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.

[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.

[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.

[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.

[6]http://2011.igem.org/Team:XMU-China