Difference between revisions of "Part:BBa K934025:Experience"

Revision as of 00:43, 26 September 2012

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Applications of BBa_K934025

To characterize the function of the Lux/Tet hybrid promoter, we constructed a part Plux/tet-GFP (BBa_ K934024) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline). In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.

User Reviews

UNIQf91c6c3bd9689295-partinfo-00000000-QINU UNIQf91c6c3bd9689295-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K934025===
+[[Image:Pluxtet_assay.png|thumb|center|500px|]]
+To characterize the function of the Lux/Tet hybrid promoter, we constructed a part Plux/tet-GFP (BBa_ K934024) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).
+In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.
 ===User Reviews===