Difference between revisions of "Part:BBa K863114:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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Cellulose binding domain form a cloning-vector. <partinfo>J61101</partinfo> and [https://parts.igem.org/Part:BBa_I13522 GFP] from the parts.igem.
+
The source of the cellulose binding domain is a cloning-vector from the fermentations group of Bielefeld University. <partinfo>J61101</partinfo> and [https://parts.igem.org/Part:BBa_I13522 GFP] are from the parts.igem.
  
 
===References===
 
===References===

Revision as of 00:30, 26 September 2012

CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 1109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1020


Design Notes

This part is was made by a mixture of Freiburg-assembly of BBa_K863111 and BBa_K863121 and suffix insertion into <partinfoBBa_J61101</partinfo>. Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.

Source

The source of the cellulose binding domain is a cloning-vector from the fermentations group of Bielefeld University. BBa_J61101 and GFP are from the parts.igem.

References