Difference between revisions of "Part:BBa K864401"
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Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein: (K6T, K7E. Y101S, C143S, M146I, S158A) | Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein: (K6T, K7E. Y101S, C143S, M146I, S158A) | ||
| − | + | Adding these following substitutions created a more thermostable and photostable product than the above product: (S3P, V19I, Y101S, M146I, N168D) | |
Revision as of 00:21, 26 September 2012
aeBlue blue chromoprotein
aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
Source
The protein was first extracted and characterized in (Shkrob et. al., 2005) under the name aeCP597. This version is codon optimized for E.coli
Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein: (K6T, K7E. Y101S, C143S, M146I, S158A)
Adding these following substitutions created a more thermostable and photostable product than the above product: (S3P, V19I, Y101S, M146I, N168D)
References
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]