Difference between revisions of "Part:BBa K750111:Design"

(→‎Design Notes)
(→‎References)
 
Line 13: Line 13:
  
 
===References===
 
===References===
 +
[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5

Latest revision as of 23:48, 25 September 2012

PtetGLT:GFP(lva) expression system controlled by (anhydro)tetracyclin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1615


Design Notes

We built this part from BBa_K145280. Compared with BBa_K145280, we changed the strength of RBS from 0.3 to 1.o. By this way, this part could be induct by atc(we don`t know why it can`t work when strength of RBS is 0.3).

Source

BBa_K145280

References

[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5