Difference between revisions of "Part:BBa K819009:Design"

 
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<partinfo>BBa_K819009 short</partinfo>
 
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===Source===
 
===Source===
  
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The promoter used here is from Partsregistry. The CheZ gene is from E.coli strain MG1655 by colony PCR and aligned with CheZ sequence at EcoGene database.  
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The promoter used here was from 2012 Spring Distribution Kit from Partsregistry. The CheZ gene was extracted from E.coli strain MG1655 by colony PCR and it is aligned with CheZ sequence at EcoGene database.
  
 
===References===
 
===References===

Revision as of 23:15, 25 September 2012

Weak cheZ generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.


Source

The promoter used here was from 2012 Spring Distribution Kit from Partsregistry. The CheZ gene was extracted from E.coli strain MG1655 by colony PCR and it is aligned with CheZ sequence at EcoGene database.

References