Difference between revisions of "Part:BBa K819009"
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<partinfo>BBa_K819009 short</partinfo>
 
<partinfo>BBa_K819009 short</partinfo>
  
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CheZ is dephosphorylase of CheY in chemotaxis pathway. CheZ ligated to a weak promoter. This part express CheZ protein weakly and will induce weak mobility in delta CheZ E.coli strain.
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CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br />
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CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23112 BBa_J23112]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
  
 
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Revision as of 23:11, 25 September 2012

Weak cheZ generator

CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23112. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]