Difference between revisions of "Part:BBa K819002:Design"

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===Design Notes===
 
===Design Notes===
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The original recA with the binding site of CTGT(N)<sub>8</sub>ACAG is mutated to CCGT(N)<sub>8</sub>ACGG, which is the 408 form.<br />The LexA 408 protein has corresponding mutations of PA40, NS41 and AS42.
+
The original recA with the binding site of CTGT(N)<sub>8</sub>ACAG is mutated to CCGT(N)<sub>8</sub>ACGG, which is the 408 form.<br />The LexA408 protein has corresponding mutations of PA40, NS41 and AS42.
  
 
===Source===
 
===Source===
  
−
The sequence of RecA was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000814&format=jsp&type=gene RegulonDB]<br />The promoter was synthesized by PCR<br />
+
The sequence of RecA was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000814&format=jsp&type=gene RegulonDB]<br />The promoter was synthesized by PCR and site directed mutagenesis<br />
  
 
===References===
 
===References===

Revision as of 22:42, 25 September 2012

Luminesensor repressible recA408 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original recA with the binding site of CTGT(N)8ACAG is mutated to CCGT(N)8ACGG, which is the 408 form.
The LexA408 protein has corresponding mutations of PA40, NS41 and AS42.

Source

The sequence of RecA was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000814&format=jsp&type=gene RegulonDB]
The promoter was synthesized by PCR and site directed mutagenesis

References