Difference between revisions of "Part:BBa K808025"
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* [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations] | * [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations] | ||
− | Our Material Science Lab performed | + | Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Material_Science Material Science Lab] performed |
*[http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy] | *[http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy] | ||
+ | |||
+ | Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Degradation Team Degradation] | ||
+ | * [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Degradation#Enzyme_Activity_Assays Enzyme activity assay] using a [http://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#pNP-Assay pNP-Assay] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 22:34, 25 September 2012
FsC: Cutinase PET cleaving enzyme
A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.
Usage and Biology
The characterization of FsC occured in 3 different ways.
Our [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Simulation Simulation Lab] performed
- [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations]
Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Material_Science Material Science Lab] performed
- [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy]
Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Degradation Team Degradation]
- [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Degradation#Enzyme_Activity_Assays Enzyme activity assay] using a [http://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#pNP-Assay pNP-Assay]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 379
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 525
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 301