Difference between revisions of "Part:BBa K782077"
UrosZupancic (Talk | contribs) |
UrosZupancic (Talk | contribs) |
||
| Line 4: | Line 4: | ||
==Introduction== | ==Introduction== | ||
| − | Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782007 NicTAL12] ([https://parts.igem.org/Part:BBa_K782003 sequence]) that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. | + | Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782007 NicTAL12] ([https://parts.igem.org/Part:BBa_K782003 binding site's sequence]) that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. |
[[Image:Nicbfp.png]] | [[Image:Nicbfp.png]] | ||
Revision as of 21:56, 25 September 2012
12x[NicTAL] operator_CMV promoter_BFP
Introduction
Our construct contain twelve binding sites for NicTAL12 (binding site's sequence) that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results:
BFP was obtained from Evrogen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 464
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 16
Illegal AgeI site found at 248 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1140
Illegal BsaI.rc site found at 1796