Difference between revisions of "Part:BBa K863006:Design"
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===Source=== | ===Source=== | ||
| − | E. coli BL21 (DE3) | + | The gene sequence was isolated from ''E. coli'' BL21(DE3). |
===References=== | ===References=== | ||
Revision as of 21:52, 25 September 2012
ecol laccase from E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 225
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'
Source
The gene sequence was isolated from E. coli BL21(DE3).