Difference between revisions of "Part:BBa K808027"
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EstA is a membrane bound but inactive esterase from ''Pseudomonas aeruginosa''. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein [https://parts.igem.org/Part:BBa_K808030 BBa_K808030](Figure 1).
 
EstA is a membrane bound but inactive esterase from ''Pseudomonas aeruginosa''. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein [https://parts.igem.org/Part:BBa_K808030 BBa_K808030](Figure 1).
  
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[[Image:pnb_EstA_ohne_mem.png|400px|center|thumb|Figure 1:[https://parts.igem.org/Part:BBa_K808030 BBa_K808030] EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane]]
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[[Image:pnb_EstA_ohne_mem.png|400px|center|thumb|Figure 1:Visualization of [https://parts.igem.org/Part:BBa_K808030 BBa_K808030]: EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane]]
  
  

Revision as of 21:48, 25 September 2012

EstA : inactive fusion protein for bacterial surface expression

EstA is a membrane bound but inactive esterase from Pseudomonas aeruginosa. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein BBa_K808030(Figure 1).

Figure 1:Visualization of BBa_K808030: EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 235
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]