Difference between revisions of "Part:BBa K782082"
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==Introduction== | ==Introduction== | ||
| − | Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782011 NicTAL12] ([https://parts.igem.org/Part:BBa_K782007 sequence]) that are cloned upstream of CMV promoter. | + | Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782011 NicTAL12] ([https://parts.igem.org/Part:BBa_K782007 sequence]) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. |
[[Image:Nicbfp2.png]] | [[Image:Nicbfp2.png]] | ||
Revision as of 21:39, 25 September 2012
12x[NicTAL] operator_CMV promoter_2xBFP:NLS
Introduction
Our construct contain twelve binding sites for NicTAL12 (sequence) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results:
BFP was obtained from Evrogen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 464
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 16
Illegal AgeI site found at 248 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1139
Illegal BsaI.rc site found at 1795
Illegal BsaI.rc site found at 2500