Difference between revisions of "Part:BBa K782074"
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<partinfo>BBa_K782074 short</partinfo> | <partinfo>BBa_K782074 short</partinfo> | ||
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| + | TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011). | ||
==Introduction== | ==Introduction== | ||
Revision as of 20:46, 25 September 2012
10x[TALA] operator_CMV promoter_BFP
TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
Introduction
Our construct contain TALA binding sites that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results:
BFP was obtained from Evrogen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 720
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 507
Illegal AgeI site found at 12
Illegal AgeI site found at 347
Illegal AgeI site found at 372
Illegal AgeI site found at 707 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1396
Illegal BsaI.rc site found at 2052