Difference between revisions of "Part:BBa K808011"
(→Usage and Biology) |
|||
| Line 11: | Line 11: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | TphA1 is just like TphA2 an rieske protein. In addition to the rieske center, TphA1 has a binding site for NADPH + H +. The catalytic domäne oxidizes NADPH+H+ and reduces TphA1. Now the reduced | + | TphA1 is just like TphA2 an rieske protein. In addition to the rieske center, TphA1 has a binding site for NADPH + H<sup>+</sup>. The catalytic domäne oxidizes NADPH + H<sup>+</sup> and reduces TphA1. Now the reduced TphA1 reduces the TphA2A3 complex. |
| Line 38: | Line 38: | ||
<partinfo>BBa_K808011 parameters</partinfo> | <partinfo>BBa_K808011 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| − | |||
==References== | ==References== | ||
Revision as of 19:24, 25 September 2012
tphA1: reduces the tphA2A3 complex
Figure 1. Homology modelling of TphA1. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphA1 click here.
TphA1 is coding for the terephthalate dioxygenase reductase from Comamonas testosteroni KF-1. TphA1 forms together with tphA2 and tphA3 the terephthalic acid 1,2-dioxigenase system (TERDOS). TERDOS catalyzes the first reaction in the terephthalic acid degradation.
Usage and Biology
TphA1 is just like TphA2 an rieske protein. In addition to the rieske center, TphA1 has a binding site for NADPH + H+. The catalytic domäne oxidizes NADPH + H+ and reduces TphA1. Now the reduced TphA1 reduces the TphA2A3 complex.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 954
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 85
Illegal NgoMIV site found at 394 - 1000COMPATIBLE WITH RFC[1000]
References
- Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.
- Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527.
- Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101.
- Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52.