Difference between revisions of "Part:BBa K741018:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp. | |
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===Source=== | ===Source=== | ||
Latest revision as of 18:44, 25 September 2012
plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1589
Illegal NheI site found at 1612 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1370
Illegal BglII site found at 1706 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2500
Design Notes
The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp.
Source
BBa_K741001 BBa_K741009 BBa_K741016