Difference between revisions of "Part:BBa K741017:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited. | |
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===Source=== | ===Source=== | ||
Latest revision as of 18:43, 25 September 2012
plac-RBS-cI-T-pRM-anticro-T-pCon(0.244)-RBS-crogfp-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1589
Illegal NheI site found at 1612 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1370
Illegal BglII site found at 1706 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2500
Design Notes
The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited.
Source
BBa_K741001 BBa_K741008 BBa_K741016