Difference between revisions of "Part:BBa K750010"
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<partinfo>BBa_K750010 short</partinfo> | <partinfo>BBa_K750010 short</partinfo> | ||
+ | == Description == | ||
This part contains BBa_K750002 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.3(BBa_K750011), TD0.01(BBa_K750012). | This part contains BBa_K750002 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.3(BBa_K750011), TD0.01(BBa_K750012). | ||
+ | |||
+ | == Performance == | ||
+ | |||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K750009 short</partinfo> | ||
+ | |||
+ | == Description == | ||
+ | This part contains BBa_K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. And this part, TD1.0, reacts faster than other circuits we designed(TD0.6:BBa_K750010,TD0.01:BBa_K750012). | ||
+ | |||
+ | == Performance == | ||
+ | |||
+ | [[Image:figuretd1.jpg|center|frame|Figure 1. The optimization of the arabinose induced concentration added into the TD1.0 including 0, 0.1, 1.0, 10 mM arabinose.]]<br> | ||
+ | [[Image:figuretd4.jpg|center|frame|Figure 2. Fluorescence curves of TD0.6 induced by 0.1mM arabinose and TD1.0 without arabinose.]]<br> | ||
+ | [[Image:figuretd3.jpg|center|frame|Figure 3. Fluorescence curves of TD0.01 induced by 0.1mM arabinose and TD0.01 without arabinose.]]<br> | ||
+ | [[Image:figuretd2.jpg|center|frame|Figure 4. Fluorescence curves of TD1.0 induced by 0.1mM arabinose and TD0.6 without arabinose]]<br> | ||
+ | [[Image:figuretd5.jpg|center|frame|Figure 5. Fluorescence curves of the induced TD0.01, TD0.6, TD1.0 and the control BL21]]<br> | ||
+ | [[Image:figuretd6.jpg|center|frame|Figure 6. Experiemently measured the average time of the three cultures’ fluorescence up to 5000 after induction.]]<br> | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 18:40, 25 September 2012
TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP
Description
This part contains BBa_K750002 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.3(BBa_K750011), TD0.01(BBa_K750012).
Performance
TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP
Description
This part contains BBa_K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. And this part, TD1.0, reacts faster than other circuits we designed(TD0.6:BBa_K750010,TD0.01:BBa_K750012).
Performance
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1072 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 796
Illegal BamHI site found at 65
Illegal BamHI site found at 1012 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2027
Illegal BsaI.rc site found at 2754