Difference between revisions of "Part:BBa K896007"

Latest revision as of 18:05, 25 September 2012

Nap( Nitrate->Nitrite reductase)

Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. Even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.

(David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson, The Prokaryotic Nitrate Reductases)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1594
Illegal PstI site found at 2427
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1594
Illegal PstI site found at 2427
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1594
Illegal BamHI site found at 1803
Illegal XhoI site found at 2361
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1594
Illegal PstI site found at 2427
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1594
Illegal PstI site found at 2427
Illegal NgoMIV site found at 2983
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1342
Illegal SapI.rc site found at 1903

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K896007 short</partinfo>
-A type of respiratory NO3-reductase is a water-soluble, periplasmicallylocated protein known as Nap. We acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774, the function of Nap is to transfer NO into nitrogen.<p></p>
+Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. Even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.
+<p></p>
 [[Image:Nap cloning.jpg]]