Difference between revisions of "Part:BBa K934022"
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<partinfo>BBa_K934022 short</partinfo> | <partinfo>BBa_K934022 short</partinfo> | ||
| − | We constructed this part by combining | + | We constructed this part by combining [https://parts.igem.org/Part:BBa_R0062 BBa_R0062] and [https://parts.igem.org/Part:BBa_K081016 BBa_K081016]. |
This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex. | This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex. | ||
| Line 9: | Line 9: | ||
In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL. | In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL. | ||
| − | We accomplished a positive feedback system with our improved part Plux-LasI(https://parts.igem.org/Part:BBa_K934012 BBa_K934012). | + | We accomplished a positive feedback system with our improved part Plux-LasI([https://parts.igem.org/Part:BBa_K934012 BBa_K934012]). |
For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki]. | For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki]. | ||
Revision as of 18:03, 25 September 2012
Plux-LasI
We constructed this part by combining BBa_R0062 and BBa_K081016. This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex.
In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL.
We accomplished a positive feedback system with our improved part Plux-LasI(BBa_K934012).
For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 745
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 307
- 1000COMPATIBLE WITH RFC[1000]