Difference between revisions of "Part:BBa K823041"

Revision as of 17:54, 25 September 2012

PydfG promoter regulated by ecf41

PydfG is a promotor from B. lincheniformes and is regulated by the alternative sigma factor ecf41

This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on.

Our team used this part in the combination with PspoIVB/PsspK,ECF41 and MazF in the Suicide switch.

Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.

This Platereader measurment is the first and not reproduced to this point!

This Platereader assay was done with the Bacillus subtilis strain W168 containing thrC::PspoIVB-ECF41 (through transformation with pSBBs4S-PspoIVB-ECF41) and sacA::pydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-PydfG). That we get a signal shows that this is active together with ECF41.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K823041 short</partinfo>
 PydfG is a promotor from B. lincheniformes and is regulated by the alternative sigma factor ecf41
+This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on.
+[[Image:SuicideSwitchgrafik.jpg|600px]]
+Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 PsspK],[https://parts.igem.org/Part:BBa_K823043 ECF41] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the <b>Suicide</b> switch.
+Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
+<b>This Platereader measurment is the first and not reproduced to this point!</b>
+[[Image:PlatereaderPspoIVBecf41PydfG.jpg|600px]]
+This Platereader assay was done with the ''Bacillus subtilis'' strain W168 containing thrC::PspoIVB-ECF41 (through transformation with pSBBs4S-PspoIVB-ECF41) and sacA::pydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-PydfG). That we get a signal shows that this is active together with [https://parts.igem.org/Part:BBa_K823043 ECF41].
 <!-- Add more about the biology of this part here