Difference between revisions of "Part:BBa K750113"
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<partinfo>BBa_K750113 short</partinfo> | <partinfo>BBa_K750113 short</partinfo> | ||
| − | + | This part contains pBADcIT(BBa_K750104) and PcIGLT(BBa_K750103). Arabinose can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 17:49, 25 September 2012
pBADcIT-PcIGLT: Pbad->cI(lva)->PcI->GFP(lva)
This part contains pBADcIT(BBa_K750104) and PcIGLT(BBa_K750103). Arabinose can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1795