Difference between revisions of "Part:BBa K823043"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823043 short</partinfo> | <partinfo>BBa_K823043 short</partinfo> | ||
| − | This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG | + | This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[Image:SuicideSwitchgrafik.jpg|600px]] | ||
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| + | Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 PsspK],[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823041 PydfG] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the <b>Suicide</b> switch. | ||
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| + | Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination. | ||
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| + | <b>This Platereader measurment is the first and not reproduced to this point!</b> | ||
| + | [[Image:PlatereaderPspoIVBecf41PydfG.jpg|600px]] | ||
| + | This Platereader assay was done with the ''Bacillus subtilis'' strain W168 containing thrC::PspoIVB-ECF41 (through transformation with pSBBs4S-PspoIVB-ECF41) and sacA::pydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-PydfG). That we get a signal shows that this is active together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823041 PydfG]. | ||
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Revision as of 17:45, 25 September 2012
alternative sigma factor ecf41
This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 443
- 1000COMPATIBLE WITH RFC[1000]