Difference between revisions of "Part:BBa K737045"
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Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device. | Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device. | ||
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===Experimental Data=== | ===Experimental Data=== | ||
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Revision as of 17:28, 25 September 2012
YtfJ_Comparator
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. This device mean to test feasibility of N/P comparator,replacing Nitrate,Phosphate inputs by IPTG,aTc Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device.
Experimental Data
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1231
Illegal NheI site found at 1254
Illegal NheI site found at 2769
Illegal NheI site found at 2792 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2432
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1181
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3581