Difference between revisions of "Part:BBa K934012"
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In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL. | In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL. | ||
| + | |||
| + | We improved a previous part Plas-LuxI.<br> | ||
| + | <ol> | ||
| + | <li> | ||
| + | [https://parts.igem.org/Part:BBa_K266000 BBa_K266000] | ||
| + | </li> | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:27, 25 September 2012
Plas-LuxI
We constructed this part by combining K649000 and K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL.
We improved a previous part Plas-LuxI.
- BBa_K266000
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 749
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sequence and Features
Assembly Compatibility: